Fast Inactivation in Shaker K+ Channels

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چکیده

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Fast Inactivation in Shaker K+ Channels

Fast inactivating Shaker H4 potassium channels and nonconducting pore mutant Shaker H4 W434F channels have been used to correlate the installation and recovery of the fast inactivation of ionic current with changes in the kinetics of gating current known as "charge immobilization" (Armstrong, C.M., and F. Bezanilla. 1977. J. Gen. Physiol. 70:567-590.). Shaker H4 W434F gating currents are very s...

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Deactivation retards recovery from inactivation in Shaker K+ channels.

In Na+ channels, recovery from inactivation begins with a delay, followed by an exponential course, and hyperpolarization shortens the delay as well as hastens the entire exponential phase. These findings have been taken to indicate that Na+ channels must deactivate to recover from inactivation, and deactivation facilitates the unbinding of the inactivating particle. In contrast, it is demonstr...

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Fast Inactivation in Shaker K 1 Channels Properties of Ionic and Gating Currents

Fast inactivating Shaker H4 potassium channels and nonconducting pore mutant Shaker H4 W434F channels have been used to correlate the installation and recovery of the fast inactivation of ionic current with changes in the kinetics of gating current known as “charge immobilization” (Armstrong, C.M., and F. Bezanilla. 1977. J. Gen. Physiol. 70:567–590.). Shaker H4 W434F gating currents are very s...

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Energetics of Shaker K channels block by inactivation peptides

A synthetic peptide of the NH2-terminal inactivation domain of the ShB channel blocks Shaker channels which have an NH2-terminal deletion and mimics many of the characteristics of the intramolecular inactivation reaction. To investigate the role of electrostatic interactions in both peptide block and the inactivation process we measured the kinetics of block of macroscopic currents recorded fro...

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Binding Site in Shaker K Channels

We have examined the interaction between TEA and K 1 ions in the pore of Shaker potassium channels. We found that the ability of external TEA to antagonize block of Shaker channels by internal TEA depended on internal K 1 ions. In contrast, this antagonism was independent of external K 1 concentrations between 0.2 and 40 mM. The external TEA antagonism of internal TEA block increased linearly w...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1998

ISSN: 0022-1295,1540-7748

DOI: 10.1085/jgp.111.5.625